The Succession of Bacterial Community Attached on Biodegradable Plastic Mulches During the Degradation in Soil

Zhicheng Ju; Xiongfeng Du; Kai Feng; Shuzhen Li; Songsong Gu; Decai Jin; Ye Deng

doi:10.3389/fmicb.2021.785737

The Succession of Bacterial Community Attached on Biodegradable Plastic Mulches During the Degradation in Soil

Front Microbiol. 2021 Dec 24:12:785737. doi: 10.3389/fmicb.2021.785737. eCollection 2021.

Authors

Zhicheng Ju^{1

2}, Xiongfeng Du^{1

2}, Kai Feng¹, Shuzhen Li¹, Songsong Gu¹, Decai Jin^{1

2}, Ye Deng^{1

2}

Affiliations

¹ CAS Key Laboratory for Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
² College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.

Abstract

Despite the increasing application of biodegradable plastic mulches (BDMs) in agriculture, the colonization and succession of the attached microbial community on BDMs during their degradation processes remain poorly characterized. Here, we buried four types of commonly used BDMs, including pure polylactic acid (PLA), pure polybutylene adipate terephthalate (PBAT), and two mixtures of PLA and PBAT (85:15 and 15:85 w/w), and one classic polyethylene (PE) mulch in soil for 5 months. Both plastic components and incubation time significantly shaped the β-diversities of microbiota on the plastic mulches (p < 0.001). Meanwhile, the microbial compositions and community structures on BDMs were significantly different from PE mulch, and when excluding PE mulch, the microbiota varied more with time than by the composition of the four BDMs. The orders Burkholderiales and Pseudonocardiales were dominant on most BDMs across different time points. The genus Ramlibacter was revealed as a common biomarker for both PLA and PBAT by random-forest model, and all biomarkers for the BDMs belonged to the dominant order Burkholderiales. In addition, degradation-related and pathogen-related functional taxa were enriched in all mulches among all 40 functional groups, while surprisingly, potential pathogens were detected at higher levels on BDMs than PE. For community assembly on all mulches, the drift and dispersal processes played more important roles than selection, and in particular, the contribution of stochastic drift increased during the degradation process of BDMs while selection decreased, while the opposite trend was observed with PE mulch. Overall, our results demonstrated some degradation species and pathogens were specifically enriched on BDMs, though stochastic processes also had important impacts on the community assembly. It suggested that, similar to conventional plastic mulch, the increased usage of BDMs could lead to potential hazards to crops and human health.

Keywords: bacteria; biodegradable plastic mulches; biodegradation; plastic; plastisphere; soil; succession of microbiota.