AuAg nanocages/graphdiyne for rapid elimination and detection of trace pathogenic bacteria

Qiang Bai; Hongyang Luo; Shugao Shi; Shen Liu; Lina Wang; Fanglin Du; Zhugen Yang; Zhiling Zhu; Ning Sui

doi:10.1016/j.jcis.2022.01.046

AuAg nanocages/graphdiyne for rapid elimination and detection of trace pathogenic bacteria

J Colloid Interface Sci. 2022 May:613:376-383. doi: 10.1016/j.jcis.2022.01.046. Epub 2022 Jan 10.

Authors

Qiang Bai¹, Hongyang Luo¹, Shugao Shi¹, Shen Liu¹, Lina Wang², Fanglin Du¹, Zhugen Yang³, Zhiling Zhu⁴, Ning Sui⁵

Affiliations

¹ College of Materials Science and Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Rd., Qingdao, Shandong 266042, China.
² College of Environment and Safety Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Rd., Qingdao, Shandong 266042, China. Electronic address: lnwang2006@163.com.
³ Cranfield Water Science Institute, Cranfield University, Milton Keynes MK43 0AL, UK.
⁴ College of Materials Science and Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Rd., Qingdao, Shandong 266042, China. Electronic address: zlzhu@qust.edu.cn.
⁵ College of Materials Science and Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Rd., Qingdao, Shandong 266042, China. Electronic address: suining@qust.edu.cn.

PMID: 35042035
DOI: 10.1016/j.jcis.2022.01.046

Abstract

We prepared a biocompatible AuAg nanocages/graphdiyne @ polyethylene glycol (AuAg/GDY@PEG) composite. The combination of AuAg and GDY to obtain a synergistically enhanced photothermal effect, and the antibacterial effect of GDY and AuAg are used in combined anti-infective therapy. The in vitro antibacterial activity of AuAg/GDY@PEG was investigated, showing an impressive broad-spectrum antibacterial activity with the killing rate > 99.999%. Based on the photothermal conversion ability of AuAg/GDY@PEG, a simple photothermal immunoassay for pathogenic bacteria was successfully established. Sandwich immune response was performed on a microporous plate, the microplate containing the antibody binds specifically to the bacterium being tested, which then binds to the material with the antibody on its surface, and the signal was a change in temperature under 808 nm near-infrared light. The limit of detection (LOD) for S. typhimurium detection is 10³ CFU mL^-1, with a range of 10³-10⁷ CFU mL^-1. This method is accurate, rapid and low-cost, which can be used for on-site detection of pathogenic bacteria in food.

Keywords: Antibacterial; Gold-silver nanocages; Graphdiyne; Immunoassay; Photothermal effect.

MeSH terms

Bacteria
Gold*
Graphite*
Limit of Detection

Substances

graphdiyne
Gold
Graphite