A multiplex RT-PCR assay for detection of emergent pepper Tsw resistance-breaking variants of tomato spotted wilt virus in South Korea

Sun-Jung Kwon; Young-Eun Cho; Hee-Seong Byun; Hae-Ryun Kwak; Jang-Kyun Seo

doi:10.1016/j.mcp.2022.101792

A multiplex RT-PCR assay for detection of emergent pepper Tsw resistance-breaking variants of tomato spotted wilt virus in South Korea

Mol Cell Probes. 2022 Feb:61:101792. doi: 10.1016/j.mcp.2022.101792. Epub 2022 Jan 15.

Authors

Sun-Jung Kwon¹, Young-Eun Cho¹, Hee-Seong Byun², Hae-Ryun Kwak², Jang-Kyun Seo³

Affiliations

¹ Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea.
² Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, Republic of Korea.
³ Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea; Department of International Agricultural Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea. Electronic address: jangseo@snu.ac.kr.

PMID: 35041994
DOI: 10.1016/j.mcp.2022.101792

Abstract

Tomato spotted wilt virus (TSWV) is a highly destructive virus for pepper. Introgression of the resistance gene Tsw in pepper is used to manage TSWV worldwide; however, the occurrence of Tsw resistance-breaking (RB) variants threatens the pepper industry. Here, we developed a multiplex reverse-transcription PCR assay for detection of recently emerged Tsw RB variants in South Korea with high specificity and sensitivity.

Keywords: Molecular diagnosis; Multiplex RT-PCR; Pepper; Resistance-breaking; Tomato spotted wilt virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Multiplex Polymerase Chain Reaction
Plant Diseases / genetics
Reverse Transcriptase Polymerase Chain Reaction
Reverse Transcription
Tospovirus* / genetics