Reproductive tissue-specific translatome of a rice thermo-sensitive genic male sterile line

Wei Liu; Jing Sun; Ji Li; Chunyan Liu; Fuyan Si; Bin Yan; Zhen Wang; Xianwei Song; Yuanzhu Yang; Yuxian Zhu; Xiaofeng Cao

doi:10.1016/j.jgg.2022.01.002

Reproductive tissue-specific translatome of a rice thermo-sensitive genic male sterile line

J Genet Genomics. 2022 Jul;49(7):624-635. doi: 10.1016/j.jgg.2022.01.002. Epub 2022 Jan 15.

Authors

Wei Liu¹, Jing Sun², Ji Li², Chunyan Liu², Fuyan Si², Bin Yan², Zhen Wang², Xianwei Song², Yuanzhu Yang³, Yuxian Zhu⁴, Xiaofeng Cao⁵

Affiliations

¹ College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China; State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
² State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
³ Department of Rice Breeding, Hunan Yahua Seed Scientific Research Institute, Changsha, Hunan 410119, China.
⁴ College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China; Institute for Advanced Studies, Wuhan University, Wuhan, Hubei 430072, China. Electronic address: zhuyx@whu.edu.cn.
⁵ State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: xfcao@genetics.ac.cn.

PMID: 35041992
DOI: 10.1016/j.jgg.2022.01.002

Abstract

Translational regulation, especially tissue- or cell type-specific gene regulation, plays essential roles in plant growth and development. Thermo-sensitive genic male sterile (TGMS) lines have been widely used for hybrid breeding in rice (Oryza sativa). However, little is known about translational regulation during reproductive stage in TGMS rice. Here, we use translating ribosome affinity purification (TRAP) combined with RNA sequencing to investigate the reproductive tissue-specific translatome of TGMS rice expressing FLAG-tagged ribosomal protein L18 (RPL18) from the germline-specific promoter MEIOSIS ARRESTED AT LEPTOTENE1 (MEL1). Differentially expressed genes at the transcriptional and translational levels are enriched in pollen and anther-related formation and development processes. These contain a number of genes reported to be involved in tapetum programmed cell death (PCD) and lipid metabolism during pollen development and anther dehiscence in rice, including several encoding transcription factors and key enzymes, as well as several long non-coding RNAs (lncRNAs) that potentially affect tapetum and pollen-related genes in male sterility. This study represents the comprehensive reproductive tissue-specific characterization of the translatome in TGMS rice. These results contribute to our understanding of the molecular basis of sterility in TGMS rice and will facilitate further genetic manipulation of TGMS rice in two-line breeding systems.

Keywords: Fertility alternation; MEL1; Reproductive tissue; TGMS rice; Translating ribosome affinity purification (TRAP); Translatome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation, Plant / genetics
Infertility*
Oryza* / genetics
Plant Breeding
Plant Infertility / genetics
Temperature