Dataset on the effects of environmentally relevant humic acid concentrations on the liver protein profile in Japanese medaka (Oryzias latipes)

Victoria V Yurchenko; Alexey A Morozov; Roman A Fedorov; Ludmila G Bakina; Victor G Zgoda; Olga V Tikhonova

doi:10.1016/j.dib.2022.107796

Dataset on the effects of environmentally relevant humic acid concentrations on the liver protein profile in Japanese medaka (Oryzias latipes)

Data Brief. 2022 Jan 5:40:107796. doi: 10.1016/j.dib.2022.107796. eCollection 2022 Feb.

Authors

Victoria V Yurchenko¹, Alexey A Morozov¹, Roman A Fedorov¹, Ludmila G Bakina², Victor G Zgoda³, Olga V Tikhonova³

Affiliations

¹ Papanin Institute for Biology of Inland Waters Russian Academy of Sciences, 109, IBIW RAS, Borok 152742 Russia.
² St Petersburg Federal Research Center of the Russian Academy of Sciences, 14th Line, 39, SPC RAS, Saint Petersburg 199178 Russia.
³ Institute of Biomedical Chemistry, Pogodinskaya St., 10, IBMC, Moscow 119121 Russia.

Abstract

The data were obtained by a label-free quantification approach from a shotgun proteomics experiment, using STrap sample processing technique for protein digestion and high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) for peptide analysis. MaxQuant data processing was used to obtain proteomics data. The dataset reflects changes in the liver protein profile of Japanese medaka exposed to 0, 5, 40 and 80 mg/L nominal concentrations of Sigma-Aldrich humic acid for 96 h. Actual concentrations of humic acid were measured using the potassium dichromate photometric method and reported in mg organic carbon/L. These proteomics data are relevant for further insights into fish stress responses to humic substances-related challenge.

Keywords: Acute exposure; Humic substances; Japanese rice fish; LFQ; Shotgun proteomics.