Dataset of Phytochemical and secondary metabolite profiling of holy basil leaf (Ocimum sanctum Linn) ethanolic extract using spectrophotometry, thin layer chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance

Ulayatul Kustiati; Hevi Wihadmadyatami; Dwi Liliek Kusindarta

doi:10.1016/j.dib.2021.107774

Dataset of Phytochemical and secondary metabolite profiling of holy basil leaf (Ocimum sanctum Linn) ethanolic extract using spectrophotometry, thin layer chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance

Data Brief. 2021 Dec 30:40:107774. doi: 10.1016/j.dib.2021.107774. eCollection 2022 Feb.

Authors

Ulayatul Kustiati¹, Hevi Wihadmadyatami², Dwi Liliek Kusindarta²

Affiliations

¹ Postgraduate Student (Veterinary Science), Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
² Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.

Abstract

Holy basil (Ocimum sanctum Linn) or known also as "kemangi" in Indonesia is a plant commonly used as a herb in Asian countries. It is also medicinal with antipyretic, anti-inflammatory, anti-cancer, and neuroprotective properties. This dataset article provides broad screening of the phytochemical component of Ocimum sanctum ethanolic extract (EEOS) as well as a secondary metabolite profile of EEOS. Analyses were done qualitatively and quantitatively using a combination of spectrophotometer, thin layer chromatography, Fourier transform infrared spectroscopy (FTIR), and ¹H-nuclear magnetic resonance (¹H-NMR). Results showed that Ocimum sanctum ethanolic extract contains phytochemical compounds, including flavonoids, phenols, tannins, saponins, alkaloids, steroids, and terpenoids. In addition, a secondary metabolite was found and classified into metabolite groups including alcohol, amine, carboxylic acid, alkane, alkene, aldehyde, phenol, ether, sulfur, halogen, benzene, nitrogen, sterol, amino acid, carbohydrate, and nitrogen.

Keywords: 1H-NMR; EEOS; FTIR; Metabolite profiling; Spectrophotometry; TLC.