Ability to detect antibodies to beak and feather disease virus in blood on filter paper decreases with duration of storage

Berta Blanch-Lázaro; Raoul F H Ribot; Mathew L Berg; Soren Alexandersen; Andrew T D Bennett

doi:10.7717/peerj.12642

Ability to detect antibodies to beak and feather disease virus in blood on filter paper decreases with duration of storage

PeerJ. 2021 Dec 20:9:e12642. doi: 10.7717/peerj.12642. eCollection 2021.

Authors

Berta Blanch-Lázaro^{1

2}, Raoul F H Ribot¹, Mathew L Berg¹, Soren Alexandersen^{2

3

4}, Andrew T D Bennett¹

Affiliations

¹ Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia.
² Geelong Centre for Emerging Infectious Diseases, Geelong, Victoria, Australia.
³ School of Medicine, Deakin University, Geelong, Victoria, Australia.
⁴ Barwon Health, Geelong, Victoria, Australia.

Abstract

Background: Beak and feather disease virus (BFDV) is a circovirus that infects captive and wild psittacine birds, and is of conservation concern. The haemagglutination inhibition (HI) assay is used to determine antibody titres against BFDV, and the use of dried blood spots (DBS) on filter paper stored at room temperature has been suggested to be an equally valid technique to the use of frozen serum. However, research on other pathogens has found variable results when investigating the longevity of antibodies stored on DBS at room temperature. Consequently, we aimed to test the temporal stability of antibodies to BFDV in DBS samples stored long-term at room temperature. A further goal was to add to the current knowledge of antibody response to naturally acquired BFDV infection in crimson rosellas (Platycercus elegans).

Methods: Blood was collected from wild P. elegans in Victoria, Australia, that had been live-trapped (n = 9) or necropsied (n = 11). BFDV virus load data were obtained from blood stored in ethanol by real-time quantitative PCR (qPCR); antibody titres were obtained by HI assay from either DBS or serum samples, which had been collected concurrently. All HI assays were performed commercially by the Veterinary Diagnostic Laboratory (VDL) in Charles Sturt University, Australia, who were blind to BFDV blood status.

Results: HI titres from DBS stored at room temperature declined significantly over time (~80 weeks). By contrast, frozen serum samples assayed after 80 weeks in storage all had high HI titres, only varying up to one dilution step from the initial HI titres obtained from DBS at 3-6 weeks after sampling. Weak HI titres from DBS samples all came back negative when the test was repeated only nine weeks later. Novel high HI titres were reported in P. elegans, and while most birds with high antibody titres had corresponding negative qPCR results, a single subadult presented with high HI titres and virus load simultaneously.

Conclusion: Detection of antibodies on filter paper stored at room temperature decreases over time, increasing the chances of false negatives in these samples, and in repeated testing of samples with weak HI titres. Consequently, serum should be the preferred sample type to use for seroepidemiological studies on BFDV in parrots and other bird species. When not possible, it may help to store DBS on filter paper at -20 °C or lower. However, prompt testing of DBS samples (e.g., <6 weeks in storage) is recommended pending further research on antibody temporal stability. We also show that P. elegans, especially adults, can produce high antibody titres against BFDV, which may help them resist infection.

Keywords: BFDV; Crimson rosella; HI; Haemagglutination inhibition; PBFD; Parrots; Platycercus elegans; Serology; Wildlife disease.

Grants and funding

This work was supported by the Australian Research Council (DP180103494), Deakin University, Holsworth Wildlife Research Endowment, Wildlife Disease Association-Australasia and Birdlife Australia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.