Discovery of potential protein biomarkers associated with sugarcane white leaf disease susceptibility using a comparative proteomic approach

Kantinan Leetanasaksakul; Sittiruk Roytrakul; Narumon Phaonakrop; Suthathip Kittisenachai; Siriwan Thaisakun; Nitiya Srithuanok; Klanarong Sriroth; Laurent Soulard

doi:10.7717/peerj.12740

Discovery of potential protein biomarkers associated with sugarcane white leaf disease susceptibility using a comparative proteomic approach

PeerJ. 2022 Jan 5:10:e12740. doi: 10.7717/peerj.12740. eCollection 2022.

Authors

Kantinan Leetanasaksakul¹, Sittiruk Roytrakul¹, Narumon Phaonakrop¹, Suthathip Kittisenachai¹, Siriwan Thaisakun¹, Nitiya Srithuanok², Klanarong Sriroth², Laurent Soulard²

Affiliations

¹ Functional Proteomics Technology, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand.
² Mitr Phol Innovation and Research Center, Khoksa-at, Phu Khiao, Chaiyaphum, Thailand.

Abstract

Sugarcane white leaf disease (SCWLD) is caused by phytoplasma, a serious sugarcane phytoplasma pathogen, which causes significant decreases in crop yield and sugar quality. The identification of proteins involved in the defense mechanism against SCWLD phytoplasma may help towards the development of varieties resistant to SCWLD. We investigated the proteomes of four sugarcane varieties with different levels of susceptibility to SCWLD phytoplasma infection, namely K88-92 and K95-84 (high), KK3 (moderate), and UT1 (low) by quantitative label-free nano-liquid chromatography-tandem mass spectrometry (nano LC-MS/MS). A total of 248 proteins were identified and compared among the four sugarcane varieties. Two potential candidate protein biomarkers for reduced susceptibility to SCWLD phytoplasma were identified as proteins detected only in UT1. The functions of these proteins are associated with protein folding, metal ion binding, and oxidoreductase. The candidate biomarkers could be useful for further study of the sugarcane defense mechanism against SCWLD phytoplasma, and in molecular and conventional breeding strategies for variety improvement.

Keywords: Candidate protein biomarkers; Phytoplasma; Proteomics; Sugarcane white leaf disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers / metabolism
Disease Susceptibility
Plant Breeding
Plant Diseases
Plant Leaves
Proteomics / methods
Saccharum* / metabolism
Tandem Mass Spectrometry

Substances

Biomarkers

Grants and funding

This work was supported by the National Science and Technology Development Agency (NSTDA), Thailand (No. P-20-52173). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.