Effects of ticagrelor on proliferation, apoptosis, and inflammatory factors of human aortic vascular smooth muscle cells through lncRNA KCNQ1OT1

Yan Wang; Jichao Jiao; Daohai Wang; Jingchao Song; Kejun Huang

Effects of ticagrelor on proliferation, apoptosis, and inflammatory factors of human aortic vascular smooth muscle cells through lncRNA KCNQ1OT1

Am J Transl Res. 2021 Dec 15;13(12):13462-13470. eCollection 2021.

Authors

Yan Wang¹, Jichao Jiao², Daohai Wang³, Jingchao Song⁴, Kejun Huang¹

Affiliations

¹ Department of Cardiology, Henan Provincial Chest Hospital Henan, China.
² Department of Neurosurgery, The 7th People's Hospital of Zhengzhou Zhengzhou, Henan, China.
³ Department of Gsatroenterology, Henan Tumor Hospital Zhengzhou, Henan, China.
⁴ Department of Cardiac Surgery, Henan Provincial Chest Hospital Henan, China.

PMID: 35035688
PMCID: PMC8748171

Abstract

Objective: This study was designed to determine the effects of ticagrelor on the proliferation and apoptosis of and inflammatory factors in human aortic vascular smooth muscle cells (HAVSMCs).

Methods: A total of 20 patients who were first diagnosed with coronary heart disease (CHD) from August 2020 to March 2021 and 20 healthy adults were enrolled into the study. Oxidized low-density lipoprotein (ox-LDL) and different concentrations of ticagrelor were applied in the treatment of HAVSMCs, and then the cell proliferation and apoptosis and the expression of apoptosis-related proteins, inflammatory factors, and IκBα in them were determined.

Results: Compared with the ox-LDL group, the OD value was significantly increased after ticagrelor treatment, and the apoptosis rate was significantly reduced (P<0.05); compared with the ox-LDL group, the B lymphoma-2 (Bcl-2) protein, IκB, KCNQ1OT1 expression in the ticagrelor group increased significantly, Bcl-2-associated X protein (Bax), monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) expression decreased significantly (P<0.05); The expression of serum KCNQ1OT1 in patients with coronary heart disease was significantly higher than that in healthy individuals (P<0.05).

Conclusion: Ticagrelor may regulate the expression of lncRNA KCNQ1OT1 and up-regulate the expression of IκBα to promote proliferation and anti-apoptosis, so as to prevent ox-LDL from oxidative damage to HAVSMCs.

Keywords: HAVSMCs; IκBα; Ticagrelor; apoptosis; inflammation; lncRNA KCNQ1OT1; proliferation.