A DEK domain-containing protein GhDEK2D mediated Gossypium hirsutum enhanced resistance to Verticillium dahliae

Plant Signal Behav. 2022 Dec 31;17(1):2024738. doi: 10.1080/15592324.2021.2024738. Epub 2022 Jan 16.

Abstract

DEK is associated with DNA replication and break repair, mRNA splicing, and transcriptional regulation, which had been studied in humans and mammals. The function of DEK in plants was poorly understood. In this study, GhDEK2D was identified in Gossypium hirsutum by genome-wide and post-translational modifications. GhDEK2D had been phosphorylated, acetylated and ubiquitylated under Verticillium dahliae (Vd) challenge. The GhDEK2D-silenced cotton decreased resistance against Vd. In GhDEK2D-silenced cotton plants, the reactive oxygen species was activated, the callose, xylogen, hypersensitive reaction (HR) and expression levels of defense-related genes were reduced. Homozygous overexpressing-GhDEK2D transgenic Arabidopsis lines were more resistant to Verticillium wilt (Vw). We propose that GhDEK2D was a potential molecular target for improving resistance to Vw in cotton.

Keywords: Dek domain containing protein; Verticillium dahliae (Vd); cotton resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis* / genetics
  • Disease Resistance / genetics
  • Gene Expression Regulation, Plant / genetics
  • Gossypium / genetics
  • Gossypium / metabolism
  • Plant Diseases / genetics
  • Verticillium*

Supplementary concepts

  • Verticillium dahliae

Grants and funding

This work was supported by the Natural Science Foundation of Henan Province (No. 212300410418), the Central Public-interest Scientific Institution Basal Research Fund (No. 1610162021031), Key Technologies R&D Program of Henan Province (No. 192102110167) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.