This study aimed to determine the influence of in ovo injection of Astragalus membranaceus polysaccharide on growth, feed consumption, feed conversion ratio, carcasses, hematology, and blood metabolites in Cobb 500 chicks. At the 7th day of incubation, a total of 250 eggs were randomly divided into five groups with 5 replications of 10 eggs of each: negative control (normal eggs), positive control (0.5 mL saline), 1.5 mg Astragalus membranaceus, 3.0 mg Astragalus membranaceus, and 4.5 mg in ovo Astragalus membranaceus injection. Live body weight and gain were not significantly (linear and quadratic) affected by in ovo injection of Astragalus membranaceus. Kidney and liver functions were influenced by in ovo injection of Astragalus membranaceus polysaccharides in broiler chickens. Antioxidant enzymes were quadratically increased with Astragalus membranaceus polysaccharides, and the highest values achieved with 4.5 mg. The MDA concentration was linearly and quadratically decreased with in ovo injection of Astragalus membranaceus polysaccharides when compared to negative control. The highest values of IgG and IgM were achieved with 1.5 mg Astragalus membranaceus polysaccharides when compared to all other groups. In conclusion, our results indicate that in ovo injection of Astragalus membranaceus polysaccharides 1.5-4.5 mg in broiler eggs significantly improved serum ALT, AST, AP, creatinine enzymes, antioxidant activity, and immune function.
Keywords: Astragalus membranaceus; Cobb; In ovo; blood metabolites; carcass; growth.