Derivation of the MUSIe002-A human embryonic stem cell line

Sujittra Khampang; Chuti Laowtammathron; Chanchao Lorthongpanich; Phatchanat Klaihmon; Pimjai Chingsuwanrote; Roungsin Choavaratana; Suphadtra Phornwilardsiri; Ketsara Sitthirit; Pimonwan Srisook; Yaowalak U-Pratya; Surapol Issaragrisil

doi:10.1016/j.scr.2022.102660

Derivation of the MUSIe002-A human embryonic stem cell line

Stem Cell Res. 2022 Mar:59:102660. doi: 10.1016/j.scr.2022.102660. Epub 2022 Jan 10.

Affiliations

¹ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
² Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Electronic address: chuti.lao@mahidol.ac.th.
³ Division of Infertility and Reproductive Biology, Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁴ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁵ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Bangkok Hematology Center, Wattanosoth Hospital, BDMS Center of Excellence for Cancer, Bangkok, Thailand.

PMID: 35033856
DOI: 10.1016/j.scr.2022.102660

Abstract

The MUSIe002-A cell line was established from in vitro fertilization of human sperm and oocytes donated for research with informed consent. This cell line exhibited normal human embryonic stem cell (hESC) characteristics, including typical cell morphology, expression of all pluripotent stem cell markers, and potential to differentiate into three germ layers. A karyotyping analysis revealed 46 XY chromosome and cells that did not have mycoplasma contamination. MUSIe002-A represents a valuable unlimited cell source and is of potential interest for human in vitro stem cell based-models, genetic modifications, and stem cell-based therapy of human disease.