Synergistic activity and immunomodulatory potential of levofloxacin and Synoeca-MP peptide against multi-resistant strains of Klebsiella pneumoniae

Jade Ormondes de Farias; Arthur Corrêa Resende Ferreira; Alessandra Godoi Cardoso Kostopoulos; Taia Maria Berto Rezende; Simoni Campos Dias

doi:10.1016/j.micpath.2022.105403

Synergistic activity and immunomodulatory potential of levofloxacin and Synoeca-MP peptide against multi-resistant strains of Klebsiella pneumoniae

Microb Pathog. 2022 Feb:163:105403. doi: 10.1016/j.micpath.2022.105403. Epub 2022 Jan 13.

Authors

Jade Ormondes de Farias¹, Arthur Corrêa Resende Ferreira², Alessandra Godoi Cardoso Kostopoulos³, Taia Maria Berto Rezende⁴, Simoni Campos Dias⁵

Affiliations

¹ Pós-graduação em Ciências da Saúde, Faculdade de Ciências de Saúde, Universidade de Brasília, Campus Darcy Ribeiro s/n - Asa Norte, Brasília, DF, Brazil.
² Pós-graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SHAN 916 Módulo B Avenida W5 - Asa Norte, Brasília, DF, Brazil.
³ Pós-graduação em Biologia Animal, Instituto de Biologia, Universidade de Brasília, Campus Darcy Ribeiro s/n - Asa Norte, Brasília, DF, Brazil.
⁴ Pós-graduação em Ciências da Saúde, Faculdade de Ciências de Saúde, Universidade de Brasília, Campus Darcy Ribeiro s/n - Asa Norte, Brasília, DF, Brazil; Pós-graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SHAN 916 Módulo B Avenida W5 - Asa Norte, Brasília, DF, Brazil; Curso de Odontologia, Universidade Católica de Brasília, QS 07 Lote 01, Brasília, DF, Brazil. Electronic address: taiambr@gmail.com.
⁵ Pós-graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SHAN 916 Módulo B Avenida W5 - Asa Norte, Brasília, DF, Brazil; Pós-graduação em Biologia Animal, Instituto de Biologia, Universidade de Brasília, Campus Darcy Ribeiro s/n - Asa Norte, Brasília, DF, Brazil.

PMID: 35033636
DOI: 10.1016/j.micpath.2022.105403

Abstract

The purpose of this article is to study the isolated and combined effect of the peptides Synoeca-MP and IDR-1018 against multi-resistant clinical isolates of K. pneumoniae (Kp2177569 - LACEN) in vitro. The bactericidal activity of the peptide Synoeca-MP in combination with three different classes of commercial antimicrobials and its immunomodulatory potential was also evaluated. Synoeca-MP showed better antimicrobial activity than IDR-1018 and presented synergistic action combined with levofloxacin. Therefore, Synoeca-MP and levofloxacin, and the combination of both, were used in subsequent analyses. In the presence of heat-killed antigens, cellular viability and TNF-α levels was maintained, the production of NO increased and a reduction in IL-10 production was observed. The synergistic antibacterial effect between Synoeca-MP and levofloxacin was effective against multidrug-resistant strains of K. pneumoniae. The association of Synoeca-MP and levofloxacin may present a low modulating action of pro and anti-inflammatory mediators, based on these results.

Keywords: Antimicrobial peptide; Immune system; Inflammatory mediators; Klebsiella pneumoniae; Synoeca-MP.

MeSH terms

Anti-Bacterial Agents / pharmacology
Klebsiella pneumoniae*
Levofloxacin* / pharmacology
Microbial Sensitivity Tests

Substances

Anti-Bacterial Agents
Levofloxacin