A ratiometric fluorescent probe for hydrogen sulfide in neat aqueous solution and its application in lysosome-targetable cell imaging

Abstract

Hydrogen sulfide (H₂S) has been recently regarded as one of the most important gasotransmitters in the metabolic system, while abnormal H₂S concentration is associated with various diseases. Although numerous fluorescent probes have been developed for the detection of cellular H₂S, only a few of them can monitor lysosomal H₂S with ratiometric fluorescent output. Here, we developed a water-soluble probe 1 toward H₂S by introducing 2,4-dinitrophenyl ether into a novel merocyanine-based dye. As expected, H₂S induced an obvious red-shift of the probe from 520 nm to 580 nm in neat aqueous solution, and this fluorescent ratiometric response is highly selective and sensitive (with the detection limit of 0.81 nM), rapid (within 10 s), and effective in a wide pH range (2.0-10.0). In particular, the probe was successfully applied for tracing H₂S in the lysosomes of living cells and in zebrafish.

Keywords: Cell imaging; Lysosome target; Ratiometric fluorescent probe; Sulfide; Water soluble.