Fungi fabrication, characterization, and anticancer activity of silver nanoparticles using metals resistant Aspergillus niger

Nguyen Thuy Lan Chi; Geetha Royapuram Veeraragavan; Kathirvel Brindhadevi; Arunachalam Chinnathambi; Saleh H Salmen; Sulaiman Ali Alharbi; Ramakrishnan Krishnan; Arivalagan Pugazhendhi

doi:10.1016/j.envres.2022.112721

Fungi fabrication, characterization, and anticancer activity of silver nanoparticles using metals resistant Aspergillus niger

Environ Res. 2022 May 15:208:112721. doi: 10.1016/j.envres.2022.112721. Epub 2022 Jan 11.

Authors

Nguyen Thuy Lan Chi¹, Geetha Royapuram Veeraragavan², Kathirvel Brindhadevi³, Arunachalam Chinnathambi⁴, Saleh H Salmen⁴, Sulaiman Ali Alharbi⁴, Ramakrishnan Krishnan⁵, Arivalagan Pugazhendhi⁶

Affiliations

¹ Van Lang School of Engineering and Technology, Van Lang University, Ho Chi Minh City, Viet Nam. Electronic address: chi.ntl@vlu.edu.vn.
² Department of Microbiology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
³ Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam; Center for Transdisciplinary Research (CFTR), Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
⁴ Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh, 11451, Saudi Arabia.
⁵ Department of Business, Harrisburg University of Science and Technology, Harrisburg, PA, 17101, USA.
⁶ School of Renewable Energy, Maejo University, Chiang Mai, 50290, Thailand; College of Medical and Health Science, Asia University, Taichung, Taiwan. Electronic address: pugal.smile@gmail.com.

PMID: 35031337
DOI: 10.1016/j.envres.2022.112721

Abstract

The purpose of this study was to assess the bio-fabrication possibilities of pre-isolated (from bauxite mine tailings) metal-tolerant Aspergillus niger biomass filtrate and the anticancer potential of synthesized silver nanoparticles (AgNPs) tested with a Human Cervical cancer cell line (HeLa cells: Henrietta Lacks cells). The nitrate reduction test demonstrated that A. niger has the ability to reduce nitrate, and filtrate derived from A. niger biomass efficiently fabricated AgNPs from AgNO₃, as demonstrated by a visible color change from pale greenish to brownish. The UV-visible spectroscopy analysis revealed an absorbance peak at 435 nm, which corresponded to the AgNPs. These AgNPs have been capped and stabilized with several functional groups related to various bioactive molecules such as aldehyde, benzene rings, aldehydic, amines, alcohols, and carbonyl stretch protein molecules. Fourier-Transform Infrared Spectroscopy (FTIR) analysis confirmed the capping and stabilizing chemical bonding pattern. Scanning Electron Microscopy (SEM) revealed that the synthesized AgNPs were spherical, with an average size of 21.38 nm. This bio-fabricated AgNPs has in-vitro anticancer potential when tested against the HeLa cell line due to its potential size and shape. At 100 g mL^-1 concentrations of this bio-fabricated AgNPs, the anticancer activity percentage was found to be 70.2%, and the IC₅₀ value was found to be 66.32 g m^-1. These findings demonstrated that the metal-tolerant A. niger cell filtrate could produce AgNPs with anticancer potential.

Keywords: AgNPs; Anticancer activity; Aspergillus niger; FTIR and STEM; HeLa cell Line.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemistry
Aspergillus niger
HeLa Cells
Humans
Metal Nanoparticles* / chemistry
Silver* / chemistry
Silver* / pharmacology
Spectroscopy, Fourier Transform Infrared

Substances

Anti-Bacterial Agents
Silver