Detection of Cell Viability via Fluorescence Labeling of Silicate Phosphor with a Low-Temperature Superlong Persistent Luminescence

Xiaotong Fan; Bitao Liu; Wenbo Chen; Zhichao Liu; Xiuxia Yang; Shuyu Tian; Wei Zeng; Wei Lu; Qi Chen; Jian Wei; Xue Yu; Jianbei Qiu; Ming Xie; Xuhui Xu

doi:10.1021/acsabm.9b00273

Detection of Cell Viability via Fluorescence Labeling of Silicate Phosphor with a Low-Temperature Superlong Persistent Luminescence

ACS Appl Bio Mater. 2019 Jun 17;2(6):2610-2616. doi: 10.1021/acsabm.9b00273. Epub 2019 May 23.

Authors

Xiaotong Fan¹, Bitao Liu², Wenbo Chen², Zhichao Liu¹, Xiuxia Yang¹, Shuyu Tian¹, Wei Zeng³, Wei Lu⁴, Qi Chen⁵, Jian Wei⁵, Xue Yu¹, Jianbei Qiu¹, Ming Xie⁶, Xuhui Xu¹

Affiliations

¹ College of Materials Science and Engineering, Kunming University of Science and Technology, Kunming 650093, P. R. China.
² Engineering Research Center of New Energy Storage Devices and Applications, Chongqing University of Arts and Sciences, Chongqing 402160, P. R. China.
³ College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, P. R. China.
⁴ University Research Facility in Materials Characterization and Device Fabrication, The Hong Kong Polytechnic University, Hong Kong, 999077P. R. China.
⁵ Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650093, P. R. China.
⁶ Kunming Institute of Precious Metals, Kunming 650093, P. R. China.

PMID: 35030715
DOI: 10.1021/acsabm.9b00273

Abstract

Cell viability detection is usually accompanied by large errors and toxicity. For the accuracy and safety of detection, it is urgent to develop a practical approach to realize the real-time monitoring of cell viability. Here, a low-temperature persistent phosphor was employed to facilitate the detection of cell viability. Stable low-temperature persistent luminescence originating from Ba₄Si₆O₁₆:Eu²⁺, Pr³⁺ (BSEP) was developed, which could be quantified to describe the viability of baby Syrian hamster kidney (BHK-21) cells. The defect state structure guarantees the excellent persistent luminescence at 193 K of more than 200 h of the as-prepared BSEP phosphor, which ensures the long-term biological application. The structural thermal stability and water tolerance of BSEP suggest the feasibility of the practical application. Our results provide the underlying insights of the low-temperature persistent phosphors for real-time biological detection.

Keywords: cell viability; low-temperature; persistent luminescence; phosphor; traps.