This study explored how manipulating the colour of training vests affects footballers' individual and collective performance during a Gk+6vs6+Gk medium-sided game. A total of 21 under-17 years old players were involved in three experimental conditions in a random order for a total of four days: i) CONTROL, two teams using two different colour vests; ii) SAME, both teams wearing blue vests; iii) MIXED, all 6 players per team wore different colour vests. Players' positional data was used to compute time-motion and tactical-related variables, while video analysis was used to collect technical variables. Further, these variables were synchronized with spatiotemporal data allowing to capture ball-related actions in a horizontal 2D plan. All variables were analysed from the offensive and defensive perspective. From the offensive perspective, players performed more and further shots to goal during the CONTROL than in SAME and MIXED (small effects) conditions, with a decreased distance to the nearest defender (small effects). While defending, results revealed lower distance to the nearest teammate (small effects) in the CONTROL than in the SAME and MIXED conditions, and higher team longitudinal synchronization (small effects). In addition, the CONTROL showed in general lower values of team width while defending than in the other 2 conditions. Overall, coaches may use the CONTROL condition to emphasize offensive performance and defensive behaviour over the longitudinal direction with increased physical demands. In turn, coaches may use the manipulation of players vests to emphasize defensive performance, as players seem to behave more cohesively under such scenarios.