Dopamine (DA) is an important neurotransmitter used for diagnosing various diseases from its abnormal concentrations in human fluids. Herein, an electrochemical sensor based on a composite of re-doped poly(N-methylaniline) (rePNMA) and modified multi-walled carbon nanotubes (fMWCNTs), termed fMWCNT-rePNMA, was developed to measure DA concentration. The successful modification of the fMWCNT surface was confirmed by Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Cyclic voltammetry (CV) displayed an excellent electrocatalytic activity of the fMWCNTs-rePNMA composite towards the oxidation of DA. The developed fMWCNTs-rePNMA composite demonstrated a broad linear range from 5 to 90 μmol L-1 with a low limit of detection (LOD) value of 2.23 μmol L-1, and a fast response with a high sensitivity of 251.5 nA μmol-1 L as determined from the calibration curve of the DA determination. In addition, the fMWCNTs-rePNMA composite selectively identified and quantified DA in the presence of ascorbic acid (AA) and uric acid (UA). Therefore, the fMWCNTs-rePNMA composite sensor shows potential to determine the level of DA in human urine.