Age and seasonal variation and establishment of reference intervals for water-soluble vitamins determined by liquid chromatography tandem mass spectrometry

Abstract

Objectives: We aimed to establish reference intervals for water-soluble vitamins determined by liquid chromatography tandem mass spectrometry to improve the diagnosis of vitamin deficiency and outcomes of associated conditions.

Methods: In this retrospective analysis of 24 810 specimens, we aimed to examine sex-, age-, and season-related variations in vitamin levels in different groups, set reference-value intervals for vitamin levels, and evaluate these reference values against those recommended by manufacturers.

Results: Levels of vitamins B₃, B₅, B₆, B₇, and B₁₂ were higher, and those of vitamins B₂, B₉, and C were lower, in men than in women. There were seasonal variations in levels of vitamins B₁, B₃, B₅, B₆, B₉, B₁₂, and C. Levels of vitamins B₁, B₂, B₃, B₅, B₆, B₇, B₉, and C differed across age groups; vitamin B₁ displayed significant differences between ages 0 to 14 years and adults compared with reference change values. The lower limits of vitamins B₁ (ages 15-100 y), B₂, B₃, B₇, and C were lower, and that of vitamin B₅ was higher, than the recommended reference values. Finally, the upper limits of vitamins B₁, B₃, B₅, B₆, and B₇ were lower than the recommended values.

Conclusions: For values obtained using liquid chromatography tandem mass spectrometry, the lower limits of reference intervals for vitamins B₁ (ages 15-100 y), B₂, B₃, B₇, and C should be lowered, that of vitamin B₅ should be raised, and the upper limits of reference intervals for vitamins B₁, B₃, B₅, B₆, and B₇ should be lowered.

Keywords: Age; Liquid chromatography tandem mass spectrometry; Reference intervals; Season; Water-soluble vitamins.