Presynaptic GABAB receptors differentially modulate GABA release from cholecystokinin and parvalbumin interneurons onto CA1 pyramidal neurons: A cell type-specific labeling and activating study

Caifeng Shao; Jiaxue Dong; Mingwei Zhao; Shenhao Liu; Xue Wang; Yang Yu; Lingyan Fang; Ziying Zhu; Qian Chen; Xu Xiao; Wei-Ning Zhang; Kun Yang

doi:10.1016/j.neulet.2022.136448

Presynaptic GABA_B receptors differentially modulate GABA release from cholecystokinin and parvalbumin interneurons onto CA1 pyramidal neurons: A cell type-specific labeling and activating study

Neurosci Lett. 2022 Feb 16:772:136448. doi: 10.1016/j.neulet.2022.136448. Epub 2022 Jan 10.

Authors

Caifeng Shao¹, Jiaxue Dong², Mingwei Zhao², Shenhao Liu³, Xue Wang³, Yang Yu³, Lingyan Fang³, Ziying Zhu², Qian Chen², Xu Xiao⁴, Wei-Ning Zhang⁴, Kun Yang⁵

Affiliations

¹ Department of Anesthesiology, Nantong Hospital of Traditional Chinese Medicine, Nantong Hospital Affiliated to Nanjing University of Traditional Chinese Medicine, Nantong, Jiangsu 226001, China; Department of Anatomy, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
² Department of Anatomy, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
³ Clinical Medicine Class 1907, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
⁴ Department of Pathophysiology, School of Medicine, JiangsuUniversity, Zhenjiang, Jiangsu 212013, China.
⁵ Department of Anatomy, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; Department of Neurology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. Electronic address: yangk@ujs.edu.cn.

PMID: 35026332
DOI: 10.1016/j.neulet.2022.136448

Abstract

Combining cell type-specific optogenetics and whole cell recordings on mouse acute hippocampal slices, we compared GABA release from cholecystokinin-expressing (CCK) and parvalbumin-expressing (PV) interneurons onto CA1 pyramidal neurons. Baclofen, a selective GABA_B receptor agonist, inhibited GABAergic synaptic transmission greater from CCK terminals, compared to that from PV terminals. The N-type calcium channels on CCK and P/Q-type calcium channels on PV terminals contributed to the GABA_B receptor-mediated inhibition, respectively. Our data thus provide direct evidence that GABA_B receptors differentially modulate GABA release from CCK and PV interneurons, adding to an increasing list of differences between these two interneuron subtypes in modulating hippocampal pyramidal neurons.

Keywords: Cholecystokinin; GABA; GABA(B) receptors; Hippocampus; Optogenetics; Parvalbumin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CA1 Region, Hippocampal / cytology
CA1 Region, Hippocampal / metabolism*
Calcium Channels, N-Type / metabolism
Cells, Cultured
Cholecystokinin / genetics
Cholecystokinin / metabolism
Interneurons / metabolism*
Mice
Parvalbumins / genetics
Parvalbumins / metabolism
Pyramidal Cells / metabolism*
Receptors, GABA-B / metabolism*
gamma-Aminobutyric Acid / metabolism*

Substances

Calcium Channels, N-Type
Parvalbumins
Receptors, GABA-B
gamma-Aminobutyric Acid
Cholecystokinin