The amount of soil contaminated with heavy metal increases due to urbanization, industrialization, and anthropogenic activities. Quinoa is considered a useful candidate in the remediation of such soil. In this pot experiment, the phytoextraction capacity of quinoa lines (A1, A2, A7, and A9) against different nickel (Ni) concentrations (0, 50, and 100 mg kg-1) were investigated. Required Ni concentrations were developed in polythene bags filled with sandy loam soil using nickel nitrate salt prior to two months of sowing and kept sealed up to sowing. Results showed that translocation of Ni increased from roots to shoots with an increase in soil Ni concentration in all lines. A2 line accumulated high Ni in leaf compared to the root as depicted by translocation factor 3.09 and 3.21 when grown at soil having 50 and 100 Ni mg kg-1, respectively. While, in the case of root, A7 accumulated high Ni followed by A9, A1, and A2, respectively. There was a 5-7% increased seed yield by 50 mg kg-1 Ni in all except A1 compared to control. However, growth and yield declined with a further increase in Ni level. The maximum reduction in yield was noticed in A9, which was strongly linked with poor physiological performance, e.g., chlorophyll a, b, and phenolic contents. Ni concentrations in the seed of all lines were within the permissible value set (67 ppm) by FAO/WHO. The result of the present study suggests that quinoa is a better accumulator of Ni. This species can provide the scope of decontamination of heavy metal polluted soil. The screened line can be used for future quinoa breeding programs for bioremediation and phytoextraction purpose.