The precise control and localization of a single entity on a stiff and rigid interface are crucial for exploring interentity interactions. A critical challenge for the precise positioning of a single entity on a substrate lies in how to construct a solid and flat interface with high positioning precision. Herein, we developed a solid DNA self-assembled nanoraft with high conformational stability by constructing a double-layer DNA origami. Compared with conventional single-layer DNA origami, the double-layer nanoraft showed higher deformation resistance capability with the nanorafts possessing higher structural integrity. Further, we analyzed the deformability of these two DNA origamis by structural reconstruction simulation, the results of which were significantly consistent with transmission electron microscopy experimental results. Using the deformation-resistant DNA self-assembled nanoraft, we achieved the precise positioning of single-stranded DNA probes on the DNA nanointerface by visualizing the AuNPs captured by the probes. Compared with the interface constructed by single-layer DNA origami, the distance between AuNPs on the two-layer DNA origami was more approximate to the theoretical value that we designed. The rigid nanointerface with high deformation resistance capability thus provides a powerful means for facilitating the self-assembly of heterogeneous elements with precisely controlled spacing and position.
Keywords: DNA assemble; DNA origami; deformation-resistance; positioning precision; rigidity.