Ammonium and nitrate affect sexually different responses to salt stress in Populus cathayana

Abstract

Nitrogen (N) fertilization is a promising approach to improve salt tolerance. However, it is poorly known how plant sex and inorganic N alter salt stress-induced Na⁺ uptake, distribution and tolerance. This study employed Populus cathayana Rehder females and males to examine sex-related mechanisms of salt tolerance under nitrate (NO₃ ^- ) and ammonium (NH₄ ⁺ ) nutrition. Males had a higher root Na⁺ efflux, lower root-to-shoot translocation of Na⁺ , and higher K⁺ /Na⁺ , which enhanced salt tolerance under both N forms compared to females. On the other hand, decreased root Na⁺ efflux and K⁺ retention, and an increased ratio of Na⁺ in leaves relative to shoots in females caused greater salt sensitivity. Females receiving NH₄ ⁺ rather than NO₃ ^- had greater net root Na⁺ uptake, K⁺ efflux, and translocation to the shoots, especially in leaves. In contrast, males receiving NO₃ ^- rather than NH₄ ⁺ had increased Na⁺ translocation to the shoots, especially in the bark, which may narrow the difference in leaf damage by salt stress between N forms despite a higher shoot Na⁺ accumulation and lower root Na⁺ efflux. Genes related to cell wall synthesis, K⁺ and Na⁺ transporters, and denaturized protein scavenging in the barks showed differential expression between females and males in response to salt stress under both N forms. These results suggested that the regulation of N forms in salt stress tolerance was sex-dependent, which was related to the maintenance of the K⁺ /Na⁺ ratio in tissues, the ability of Na⁺ translocation to the shoots, and the transcriptional regulation of bark cell wall and proteolysis profiles.