A Mitochondria-Targeted Ratiometric Fluorescent pH Probe

Xiangjun Liu; Linlin Wang; Tao Bing; Nan Zhang; Dihua Shangguan

doi:10.1021/acsabm.9b00061

A Mitochondria-Targeted Ratiometric Fluorescent pH Probe

ACS Appl Bio Mater. 2019 Mar 18;2(3):1368-1375. doi: 10.1021/acsabm.9b00061. Epub 2019 Mar 7.

Authors

Xiangjun Liu^{1

2}, Linlin Wang^{1

2}, Tao Bing^{1

2}, Nan Zhang^{1

2}, Dihua Shangguan^{1

2}

Affiliations

¹ Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
² University of Chinese Academy of Sciences, Beijing 100049, China.

PMID: 35021383
DOI: 10.1021/acsabm.9b00061

Abstract

The pH of mitochondria (pH_m) is crucial in physiological processes. Here, we report a new mitochondria-targeted ratiometric pH-sensitive fluorescent probe for monitoring the mitochondrial pH change. This probe, IR-ANNA, was constructed by coupling a pH-sensitive naphthalimide derivative ANNA and a mitochondria-targeted pH-insensitive cyanine dye. IR-ANNA showed good mitochondria-targeted ability, excellent pH response, and low cytotoxicity, which makes IR-ANNA suitable for monitoring pH_m in living cells. Moreover, IR-ANNA has been successfully applied for monitoring the pH change of mitochondria under the stimuli of FCCP, NAC, and H₂O₂ in living cells.

Keywords: cyanine; mitochondrial targeting; naphthalimide; pH imaging; ratiometric fluorescent probe.