Novel Centromeric and Subtelomeric Repetitive DNA Sequences for Karyotyping the Bambara Groundnut (Vigna subterranea L. Verdc.)

Ahmet L Tek; Sevim D Kara Öztürk; Hümeyra Yıldız; Didem Karalar

doi:10.1159/000521398

Novel Centromeric and Subtelomeric Repetitive DNA Sequences for Karyotyping the Bambara Groundnut (Vigna subterranea L. Verdc.)

Cytogenet Genome Res. 2021;161(12):585-594. doi: 10.1159/000521398. Epub 2022 Jan 12.

Authors

Ahmet L Tek¹, Sevim D Kara Öztürk¹, Hümeyra Yıldız¹, Didem Karalar¹

Affiliation

¹ Department of Agricultural Genetic Engineering, Ayhan Şahenk Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, Niğde, Turkey.

PMID: 35021180
DOI: 10.1159/000521398

Abstract

Bambara groundnut (Vigna subterranea L. Verdc.) is an un-derutilized minor legume crop with climate resilience and great potential use in world agriculture. This study aimed to cytogenetically characterize the genome and chromosome properties of Bambara groundnut. We cloned, sequenced, and mapped a 50-bp centromere-specific tandem repeat on all chromosomes. In addition, a 400-bp subtelomeric repeat was discovered and mapped on a single pair of chromosomes. A Bambara groundnut karyotype was constructed using these novel repeats along with ribosomal RNA genes (45S and 5S) and telomeric DNA sequences. This study provides the first analysis of the genome and chromosome properties of Bambara groundnut. We discuss our findings in relation to genetic improvement of Bambara groundnut and centromere evolution in legume species.

Keywords: Bambara groundnut; Centromeric DNA; Repetitive DNA; Subtelomere; TAREAN.

MeSH terms

Base Sequence
Centromere / genetics*
Chromosomes, Plant
Evolution, Molecular*
Genes, rRNA
Genome, Plant
Karyotyping*
Tandem Repeat Sequences / genetics*
Telomere / genetics*
Vigna / genetics*