Fibrous biotextiles are very popular structural forms that are widely used in medical products and devices ranging from sutures, bandages, wound dressing, and patches to all kinds of artificial grafts such as ligaments, tendons, blood vessels, heart valves, and tissue engineered scaffolds. Blow-spinning is a recently developed technique that enables the large-scale and efficient production of ultrathin fibers with diameters ranging from micrometer to nanometer. In this study, the blow-spinning process and parameters were optimized to steadily fabricate collagen nanofibers by ejecting a collagen solution with constant airflow with precisely controlled diameter and alignment. Different from the electrospun collagen nanofibrous membrane, the blow-spun one was fluffy and spongy with high porosity. It was observed that the blow-spun collagen membrane could better maintain the fiber structure after chemical crosslinking in comparison with the electrospun membrane crosslinked in the same condition, which probably attributed to the good porosity and permeability of crosslinking agent within the membranes. The in vitro cell culture of Schwann cells on the blow-spun collagen nanofibrous spongy membrane showed its good biocompatibility for cell attachment, growth, and migration into the membrane, implying its potential in biomedical applications. Besides, there is no requirement for electroconductivity of the polymer solution and collector in blow-spinning. In brief, our results indicated that blow-spinning is an accessible and efficient technique to prepare nanofibers of synthetic and natural polymers, which has a great prospect in the large-scale production of biotextile medical devices and tissue engineered scaffolds. Impact statement Solution blow-spinning is a recently developed fiber fabrication technology with efficient and large-scale production. In this study, we successfully prepared collagen nanofibrous membrane with precisely controlled diameter and alignment by blow-spinning. The blow-spun collagen nanofibrous spongy membrane could better maintain the fiber structure after chemical crosslinking, which showed good biocompatibility for cell spreading and migration inward.
Keywords: blow-spinning; collagen; crosslinking; nanofiber; tissue engineering.