Ternary Composite Nanofibers Containing Chondroitin Sulfate Scavenge Inflammatory Chemokines from Solution and Prohibit Squamous Cell Carcinoma Migration

William S Boyle; Weili Chen; Astrid Rodriguez; Samantha Linn; Jakub Tolar; Karen Lozano; Theresa M Reineke

doi:10.1021/acsabm.8b00690

Ternary Composite Nanofibers Containing Chondroitin Sulfate Scavenge Inflammatory Chemokines from Solution and Prohibit Squamous Cell Carcinoma Migration

ACS Appl Bio Mater. 2019 Feb 18;2(2):619-624. doi: 10.1021/acsabm.8b00690. Epub 2019 Feb 5.

Authors

William S Boyle¹, Weili Chen², Astrid Rodriguez³, Samantha Linn¹, Jakub Tolar², Karen Lozano³, Theresa M Reineke¹

Affiliations

¹ Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, United States.
² Stem Cell Institute and Division of Pediatric Blood and Marrow Transplantation, University of Minnesota, Minneapolis, Minnesota 55455, United States.
³ Department of Mechanical Engineering, University of Texas-Rio Grande Valley, Edinburg, Texas 78539, United States.

PMID: 35016300
DOI: 10.1021/acsabm.8b00690

Abstract

Recessive dystrophic epidermolysis bullosa (RDEB), an inherited disease featuring blistering wounds, causes constant inflammation that leads to the eventual development of an aggressive form of squamous cell carcinoma (RDEB SCC). The persistence of inflammatory chemokines such as MCP-1 and Il-8 in RDEB wounds may foster RDEB SCC carcinogenesis. We report the production of ternary composite nanofibers containing pullulan, chondroitin sulfate, and tannic acid as RDEB wound dressings. The swellable fibers are stable to hydration and absorb ∼500% their weight in water. The fibers remove ∼99% of MCP-1 from solution in <2 h. Scavenged media did not promote RDEB SCC migration.

Keywords: Forcespinning; carbohydrates; epidermolysis bullosa; nanofibers; wound healing.