Ursolic acid (UA) is a naturally occurring pentacyclic triterpene widely distributed in fruits and plants. It is pharmacologically active and has the potential to be a useful therapeutic compound. To date, bioanalysis of UA has been limited by biomatrix interference and poor collision induced dissociation (CID) efficiency in tandem mass spectrometry. In this study, we developed a method based on liquid chromatography differential mobility spectrometry tandem mass spectrometry LC-DMS-MS/MS with multiple ion monitoring (MIM) for quantitation of UA in rat plasma. The method involves efficient sample preparation by solid phase extraction and requires only a limited volume of plasma (40 μL) to achieve linearity in the 1-100 ng/mL range with good accuracy and precision. The method was successfully applied to a pharmacokinetic study of orally administered UA in rat. The results indicate that LC-DMS-MS/MS with MIM is a useful strategy for the bioassay of UA suitable for high throughput analysis.
Keywords: Bioanalysis; Differential mobility spectrometry; Multiple ion monitoring; Pharmacokinetics; Rat; Ursolic acid.
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