Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing

Cells. 2021 Dec 29;11(1):98. doi: 10.3390/cells11010098.

Abstract

Background: Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD).

Methods: Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD.

Results: Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p.

Conclusion: We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD.

Keywords: coronary artery disease; exosomes; micro RNAs; next-generation sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Coronary Artery Disease / genetics*
  • Exosomes / genetics*
  • Female
  • High-Throughput Nucleotide Sequencing*
  • Humans
  • Linear Models
  • Male
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Middle Aged

Substances

  • MicroRNAs