Specific detection of acetamiprid with aptamer based on flexible and adhesive SERS membrane

Jie Zhou; Dan Wang; Haifeng Yang; Feng Wang

doi:10.1016/j.saa.2021.120801

Specific detection of acetamiprid with aptamer based on flexible and adhesive SERS membrane

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Apr 5:270:120801. doi: 10.1016/j.saa.2021.120801. Epub 2021 Dec 24.

Authors

Jie Zhou¹, Dan Wang¹, Haifeng Yang², Feng Wang³

Affiliations

¹ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China.
² The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China. Electronic address: haifengyang@yahoo.com.
³ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China. Electronic address: wangfeng@shnu.edu.cn.

PMID: 35007904
DOI: 10.1016/j.saa.2021.120801

Abstract

Acetamiprid poses a serious threat to human beings. In this work, the highly transparent fluorescent quantitative PCR sealing membranes (PCR-M) developed in our previous work which had attractive characteristics such as sensitivity, stability and reproducibility, and its capability of extracting analytes quickly, was used to detect acetamiprid. The detection limit of 1 nmol/L was obtained by direct detection of CN group in acetamiprid. In order to improve the selectivity and sensitivity, acetamiprid aptamer was introduced. After the interaction of aptamer with acetamiprid the SERS signal of adenine at 730 cm^-1 increased. The detection limit of indirect detection was 10^-8 mol/L. Aptamer advanced Ag-PCR-M-based Surface enhanced Raman spectroscopy (SERS) method for indirect SERS detection which significantly improves the detection sensitivity and greatly increases the range of signal response value. This will be conducive to the detection of actual samples and expand the practical application.

Keywords: Acetamiprid detection; Aptamer; High sticky and flexible SERS substate; Surface enhanced Raman spectroscopy (SERS).

MeSH terms

Adhesives
Aptamers, Nucleotide*
Humans
Limit of Detection
Metal Nanoparticles*
Neonicotinoids
Reproducibility of Results
Spectrum Analysis, Raman

Substances

Adhesives
Aptamers, Nucleotide
Neonicotinoids
acetamiprid