Microplastic (MP) pollution in the environment is increasing, leading to growing concerns about human exposures and the subsequent impact on health. Although marine MP research has received significant attention in recent years, only a few studies have attempted characterization of MP in air and examined the MP uptake and influence via inhalation on human health. Moreover, the methods used for MP characterization in the marine environment require further optimization to be applicable to MP in the air. This paper details method for collecting and characterizing MP < 2.5 μm in air samples for the purposes of toxicological assessment. The first phase of the study evaluated (a) the suitability of various filter types to collect respirable airborne MP <2.5 μm, and; (b) the ability of Raman and enhanced darkfield-hyperspectral spectroscopy methods to identify MP reference standards collected from spiked filters and in cells after exposure to reference MP. In the second phase, these methods were employed to characterize MP <2.5 μm in personal, indoor and outdoor filter air samples and in cells following exposure to filter extracted material. The results showed the presence of a variety of MP in the respirable size fraction (0.1-1 µm aerodynamic diameter). Silver membrane filters were found not suitable for collecting and analyzing MP <2.5 μm. While it was easy to detect reference MP in cells post-exposure, the identity of only two types of air-borne MP was confirmed in cells. The study highlighted possible sources of artifacts and inconsistencies in analyzing airborne MP.
Keywords: Air pollution; Raman spectroscopy; enhanced darkfield hyperspectral imaging.