Amphiphilic Poly(N-Vinylpyrrolidone) Nanoparticles Loaded with DNA Plasmids Encoding Gn and Gc Glycoproteins of the Rift Valley Fever Virus: Preparation and In Vivo Evaluation

ACS Appl Bio Mater. 2021 Aug 16;4(8):6084-6092. doi: 10.1021/acsabm.1c00426. Epub 2021 Jul 10.

Abstract

The aim of the study was to develop amphiphilic poly(N-vinylpyrrolidone) (PVP) nanoparticles (NPs) loaded with DNA plasmids encoding Gn and Gc glycoproteins of the Rift Valley fever virus (RVFV) and to study the humoral response in vivo. DNA plasmids were protected from extracellular nucleases by loading in NPs from PVP derivatives modified with amino acids β-alanine (Ala7-PVPOD4000) or glycine (Gly7.5-PVP-OD4000) fabricated by the original self-assembly technique. The obtained NPs were administered in mice and the enhancement of humoral response compared to this one in case of immunization with native DNA plasmids was demonstrated. The NPs loaded with DNA plasmids are promising for the fabrication of various DNA particulate vaccines.

Keywords: DNA vaccines; Gn and Gc glycoproteins; Rift Valley fever virus; amphiphilic polymers; humoral response; nanoparticles; poly(N-vinylpyrrolidone).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / genetics
  • DNA
  • Glycoproteins / genetics
  • Mice
  • Mice, Inbred BALB C
  • Nanoparticles*
  • Plasmids / genetics
  • Pyrrolidinones
  • Rift Valley Fever*
  • Rift Valley fever virus* / genetics
  • Vaccines, DNA*

Substances

  • Antibodies, Viral
  • Glycoproteins
  • Pyrrolidinones
  • Vaccines, DNA
  • N-vinyl-2-pyrrolidinone
  • DNA