Fluorescent sensing of temperature in nanoscale regions has many advantages and applications in the biological field. Herein, blue emitting carbon dots (CDs) are designed and successfully developed using a one step hydrothermal method. As synthesized CDs exhibit temperature dependent photoluminescent (PL) intensity and PL decay lifetime over the physiological temperature ranging from room temperature (RT) to 70 °C. The PL intensity and PL decay lifetime of the obtained CDs correlate linearly to temperature (RT-70 °C) with correlation coefficient of 0.997 and 0.996, respectively. Additionally, dual mode thermal sensing (PL intensity/lifetime) make these CDs a promising optical nanothermometer over alternative semiconductors quantum dots and CD-based quantum dots. Moreover, the resultant aqueous CDs demonstrate excitation-independent blue emission, and the PL quantum yield (QY) is reached at 44.5%. The obtained CDs illustrate stable performance to high ionic environments and photobleaching even after keeping them for 2 h under continues UV irradiation. Furthermore, blue emitting CDs have low cytotoxicity for T-ca. cells and illuminate deep blue fluorescence under the excitation of 406 nm. As a result, high thermal sensitivity of these fluorescent CDs has potential to detect temperature in living cells in the range of 25-40 °C.
Keywords: bioimaging; blue emission; carbon dots; nanothermal sensors; temperature dependent fluorescence.