Intraoperative colon perfusion assessment using multispectral imaging

Neil T Clancy; António S Soares; Sophia Bano; Laurence B Lovat; Manish Chand; Danail Stoyanov

doi:10.1364/BOE.435118

Intraoperative colon perfusion assessment using multispectral imaging

Biomed Opt Express. 2021 Nov 12;12(12):7556-7567. doi: 10.1364/BOE.435118. eCollection 2021 Dec 1.

Authors

Neil T Clancy^{1

2}, António S Soares^{1

3}, Sophia Bano^{1

4}, Laurence B Lovat^{1

3}, Manish Chand^{1

3}, Danail Stoyanov^{1

4}

Affiliations

¹ Wellcome/EPSRC Centre for Interventional and Surgical Sciences, University College London, UK.
² Department of Medical Physics and Biomedical Engineering, University College London, UK.
³ Division of Surgery and Interventional Sciences, University College London, UK.
⁴ Department of Computer Science, University College London, UK.

Abstract

In colorectal surgery an anastomosis performed using poorly-perfused, ischaemic bowel segments may result in a leak and consequent morbidity. Traditional measures of perfusion assessment rely on clinical judgement and are mainly subjective, based on tissue appearance, leading to variability between clinicians. This paper describes a multispectral imaging (MSI) laparoscope that can derive quantitative measures of tissue oxygen saturation (SO₂ ). The system uses a xenon surgical light source and fast filter wheel camera to capture eight narrow waveband images across the visible range in approximately 0.3 s. Spectral validation measurements were performed by imaging standardised colour tiles and comparing reflectance with ground truth spectrometer data. Tissue spectra were decomposed into individual contributions from haemoglobin, adipose tissue and scattering, using a previously-developed regression approach. Initial clinical results from seven patients undergoing colorectal surgery are presented and used to characterise measurement stability and reproducibility in vivo. Strategies to improve signal-to-noise ratio and correct for motion are described. Images of healthy bowel tissue (in vivo) indicate that baseline SO₂ is approximately 75 ± 6%. The SO₂ profile along a bowel segment following ligation of the inferior mesenteric artery (IMA) shows a decrease from the proximal to distal end. In the clinical cases shown, imaging results concurred with clinical judgements of the location of well-perfused tissue. Adipose tissue, visibly yellow in the RGB images, is shown to surround the mesentery and cover some of the serosa. SO₂ in this tissue is consistently high, with mean value of 90%. These results show that MSI is a potential intraoperative guidance tool for assessment of perfusion. Mapping of SO₂ in the colon could be used by surgeons to guide choice of transection points and ensure that well-perfused tissue is used to form an anastomosis. The observation of high mesenteric SO₂ agrees with work in the literature and warrants further exploration. Larger studies incorporating with a wider cohort of clinicians will help to provide retrospective evidence of how this imaging technique may be able to reduce inter-operator variability.

Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.