Inhibition of cadmium uptake by wheat with urease-producing bacteria combined with sheep manure under field conditions

Hui Han; Xuejiao Wu; Nanthi Bolan; M B Kirkham; Jianjun Yang; Zhaojin Chen

doi:10.1016/j.chemosphere.2022.133534

Inhibition of cadmium uptake by wheat with urease-producing bacteria combined with sheep manure under field conditions

Chemosphere. 2022 Apr:293:133534. doi: 10.1016/j.chemosphere.2022.133534. Epub 2022 Jan 6.

Authors

Hui Han¹, Xuejiao Wu², Nanthi Bolan³, M B Kirkham⁴, Jianjun Yang⁵, Zhaojin Chen²

Affiliations

¹ Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China; Collaborative Innovation of Water Security for the Water Source Region of Mid-line of the South-to-North Diversion Project of Henan Province, College of Life Sciences and Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, PR China.
² Collaborative Innovation of Water Security for the Water Source Region of Mid-line of the South-to-North Diversion Project of Henan Province, College of Life Sciences and Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, PR China.
³ Global Centre for Environmental Remediation, University of Newcastle, Callaghan Campus, NSW, 2308, Australia.
⁴ Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA.
⁵ Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China. Electronic address: yangjianjun@caas.cn.

PMID: 34999099
DOI: 10.1016/j.chemosphere.2022.133534

Abstract

In heavy metal-contaminated farmland, microorganisms or organic fertilizers can be used to minimize heavy metal uptake by crops to ensure food safety. However, the mechanisms by which urease-producing and metal-immobilizing bacteria combined with manure inhibit Cd uptake in wheat (Triticum aestivum L.) remain unclear. Herein, the effects of Enterobacter bugandensis TJ6, sheep manure (SM), and TJ6 combined with SM on Cd uptake by wheat and the mechanisms involved were investigated under field conditions. The results showed that strain TJ6 increased the urease activity and the proportion of strains with a high Cd adsorption capacity in SM, thereby enhancing the Cd adsorption capacity of SM in solution. Strain TJ6 combined with SM improved the rhizosphere soil urease activity, NH₄⁺/NO₃^- ratio, and pH, thus reducing the Cd content (75.9%) in wheat grain. In addition, TJ6+SM reduced the bacterial community diversity but shifted the structure of the bacterial community in rhizosphere soil. Interestingly, the relative abundances of urease-producing bacteria and metal-immobilizing bacteria (Enterobacter, Bacillus, Exiguobacterium, Rhizobium, and Serratia) in rhizosphere soil were enriched, which enhanced wheat resistance to Cd toxicity. These results showed that urease-producing and metal-immobilizing bacteria combined with sheep manure can inhibit the uptake of Cd by wheat.

Keywords: Field experiment; Immobilization; Sheep manure; Urease-producing bacteria; Wheat.

MeSH terms

Animals
Cadmium / analysis
Cadmium / toxicity
Manure
Metals, Heavy* / analysis
Metals, Heavy* / toxicity
Sheep
Soil / chemistry
Soil Pollutants* / analysis
Soil Pollutants* / toxicity
Triticum
Urease

Substances

Manure
Metals, Heavy
Soil
Soil Pollutants
Cadmium
Urease