Hydrogen peroxide can be a plausible biomarker in cyanobacterial bloom treatment

Abstract

The effect of combined stresses, photoinhibition, and nutrient depletion on the oxidative stress of cyanobacteria was measured in laboratory experiments to develop the biomass prediction model. Phormidium ambiguum was exposed to various photosynthetically active radiation (PAR) intensities and phosphorous (P) concentrations with fixed nitrogen concentrations. The samples were subjected to stress assays by detecting the hydrogen peroxide (H₂O₂) concentration and antioxidant activities of catalase (CAT) and superoxide dismutase (SOD). H₂O₂ concentrations decreased to 30 µmol m^-2 s^-1 of PAR, then increased with higher PAR intensities. Regarding P concentrations, H₂O₂ concentrations (nmol L^-1) generally decreased with increasing P concentrations. SOD and CAT activities were proportionate to the H₂O₂ protein^-1. No H₂O₂ concentrations detected outside cells indicated the biological production of H₂O₂, and the accumulated H₂O₂ concentration inside cells was parameterized with H₂O₂ concentration protein^-1. With over 30 µmol m^-2 s^-1 of PAR, H₂O₂ concentration protein^-1 had a similar increasing trend with PAR intensity, independently of P concentration. Meanwhile, with increasing P concentration, H₂O₂ protein^-1 decreased in a similar pattern regardless of PAR intensity. Protein content decreased with gradually increasing H₂O₂ up to 4 nmol H₂O₂ mg^-1 protein, which provides a threshold to restrict the growth of cyanobacteria. With these results, an empirical formula-protein (mg L^-1) = - 192*Log((H₂O₂/protein)/4.1), where H₂O₂/protein (nmol mg^-1) = - 0.312*PAR²/(50² + PAR²)*((25/PAR)⁴ + 1)*Log(P/133,100), as a function of total phosphorus concentration, P (µg L^-1)-was developed to obtain the cyanobacteria biomass.