Feature-based molecular networking-guided discovery of siderophores from a marine mesophotic zone Axinellida sponge-associated actinomycete Streptomyces diastaticus NBU2966

Yang Liu; Lijian Ding; Yueting Deng; Xiao Wang; Wei Cui; Shan He

doi:10.1016/j.phytochem.2021.113078

Feature-based molecular networking-guided discovery of siderophores from a marine mesophotic zone Axinellida sponge-associated actinomycete Streptomyces diastaticus NBU2966

Phytochemistry. 2022 Apr:196:113078. doi: 10.1016/j.phytochem.2021.113078. Epub 2022 Jan 4.

Authors

Yang Liu¹, Lijian Ding², Yueting Deng¹, Xiao Wang³, Wei Cui⁴, Shan He⁵

Affiliations

¹ Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, Zhejiang, China.
² Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, Zhejiang, China. Electronic address: dinglijian@nbu.edu.cn.
³ Immunology Innovation Team, School of Medicine, Ningbo University, Ningbo 315211, Zhejiang, China.
⁴ Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo 315200, Zhejiang, China.
⁵ Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, Zhejiang, China; Ningbo Institute of Marine Medicine, Peking University, Ningbo 315800, Zhejiang, China. Electronic address: heshan@nbu.edu.cn.

PMID: 34995882
DOI: 10.1016/j.phytochem.2021.113078

Abstract

Ten siderophore-related compounds were isolated by MS/MS-based molecular networking from a mesophotic sponge-associated Streptomyces diastaticus NBU2966. Among them, there were three kinds of siderophores including three undescribed phenol/thiazoline-type compounds, thiazostatin C, methyl thiazostatin B, and pulicatin J, one undescribed phenol/oxazoline-type compound, spoxazomicin E, and one undescribed phenol/oxazoline/thiazoline-type compound, streptochelin A, as well as five known compounds. Pulicatin J and spoxazomicin E were both isolated as a pair of inseparable epimers. The planar structures of all these compounds were determined based on the detailed NMR and HRESIMS spectroscopic analysis, and the absolute configurations of them were assigned by ECD and NMR quantum chemical calculations. Additionally, in vitro antibacterial activities, inhibition of MRSA biofilm formation, and neuroprotective activity for these compounds were tested.

Keywords: Mesophotic coral ecosystems; Molecular networking; Siderophores; Streptomyces diastaticus; Streptomycetaceae.

MeSH terms

Actinobacteria*
Molecular Structure
Siderophores / chemistry
Streptomyces* / chemistry
Tandem Mass Spectrometry

Substances

Siderophores

Supplementary concepts

Streptomyces diastaticus