Generation of a multi-herbicide-tolerant alfalfa by using base editing

Emilia Bottero; Cristina Gómez; Margarita Stritzler; Hiromi Tajima; Romina Frare; Cecilia Pascuan; Eduardo Blumwald; Nicolás Ayub; Gabriela Soto

doi:10.1007/s00299-021-02827-w

Generation of a multi-herbicide-tolerant alfalfa by using base editing

Plant Cell Rep. 2022 Feb;41(2):493-495. doi: 10.1007/s00299-021-02827-w. Epub 2022 Jan 7.

Authors

Emilia Bottero^{1

2}, Cristina Gómez^{1

2}, Margarita Stritzler^{1

2}, Hiromi Tajima³, Romina Frare^{1

2}, Cecilia Pascuan^{1

2}, Eduardo Blumwald³, Nicolás Ayub^{1

2}, Gabriela Soto^{4

5}

Affiliations

¹ Instituto de Agrobiotecnología y Biología Molecular, CONICET-INTA, Buenos Aires, Argentina.
² Instituto de Genética "Edwald Alfredo Favret", INTA, De los Reseros S/N, C25 (1712), Castelar, Argentina.
³ Department of Plant Sciences, University of California, Davis, CA, 95616, USA.
⁴ Instituto de Agrobiotecnología y Biología Molecular, CONICET-INTA, Buenos Aires, Argentina. soto.gabrielacinthia@inta.gob.ar.
⁵ Instituto de Genética "Edwald Alfredo Favret", INTA, De los Reseros S/N, C25 (1712), Castelar, Argentina. soto.gabrielacinthia@inta.gob.ar.

PMID: 34994854
DOI: 10.1007/s00299-021-02827-w

Abstract

We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides.

Keywords: ALS; Alfalfa; Base editing; CRISPR/Cas9; Herbicides.

MeSH terms

Gene Editing / methods*
Herbicide Resistance / genetics
Herbicides / chemistry
Herbicides / pharmacology*
Medicago sativa / drug effects*
Medicago sativa / genetics*
Plants, Genetically Modified
Sulfonylurea Compounds / pharmacology

Substances

Herbicides
Sulfonylurea Compounds

Abstract

MeSH terms

Substances

Grants and funding