The mitochondrial genomes of two Gryllus crickets (Grylloidea: Gryllidae) via RNA-seq

Alex S Torson; Alexandra M A Hicks; Claire E Baragar; David R Smith; Brent J Sinclair

doi:10.1080/23802359.2021.2010613

The mitochondrial genomes of two Gryllus crickets (Grylloidea: Gryllidae) via RNA-seq

Mitochondrial DNA B Resour. 2021 Dec 23;7(1):106-107. doi: 10.1080/23802359.2021.2010613. eCollection 2022.

Authors

Alex S Torson¹, Alexandra M A Hicks¹, Claire E Baragar¹, David R Smith¹, Brent J Sinclair¹

Affiliation

¹ Department of Biology, The University of Western Ontario, London, ON, Canada.

Abstract

Here, we used RNA-seq reads to assemble the complete mitochondrial genomes of the spring field cricket, Gryllus veletis, and the variable field cricket, Gryllus lineaticeps. The mitochondrial genomes of G. veletis (15,686 bp, MW322713) and G. lineaticeps (15,607 bp, MW315773) each contain the expected 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a large control (D-loop) region. The arrangements of these features were similar for both species and consistent with other closely related Orthoptera. A phylogenetic analysis of the mitochondrial genome sequences revealed that G. veletis and G. lineaticeps cluster with the other Gryllus species and all reside in a clade with the Gryllidae.

Keywords: Gryllus lineaticeps; Gryllus veletis; RNA-seq; mitochondrial genome sequence.

Grants and funding

This project was supported by a Natural Sciences and Engineering Research Council (NSERC) Discovery Grant to BJS, and an NSERC Undergraduate Student Research Award to CEB.