Kaistella soli sp. nov., isolated from oil-contaminated experimental soil

Abstract

A light yellow-coloured, non-motile, aerobic, Gram-stain-negative, and rod-shaped bacterial strain DKR-2^T was isolated from oil-contaminated experimental soil. The strain was catalase and oxidase positive, and grew at 0-1.5% (w/v) NaCl concentration, at temperature 10-35 °C, and at pH 6.0-9.5. The phylogenetic analysis suggested that the strain DKR-2^T was affiliated to the genus Kaistella, with the closest species being Kaistella haifensis DSM 19056^T (97.6% 16S rRNA gene sequence similarity). The principle fatty acids were iso-C_15:0, summed feature 9 (iso-C_17:1 ω9c and/or C_16:0 10-methyl), and antiso-C_15:0. The sole menaquinone was MK-6 and major polar lipid was phosphatidylethanolamin. The DNA G+C content was 39.5%. The dDDH (in silico DNA-DNA hybridization) and ANI (average nucleotide identity) values between strain DKR-2^T and K. haifensis DSM 19056^T were 22.4% and 79.3%, respectively. In addition, both dDDH and ANI values between strain DKR-2^T and other phylogenetically related neighbours were < 25.0% and < 77.0%, respectively. In overall, the polyphasic taxonomic data presented in this study clearly indicated that strain DKR-2^T represents a novel species in the genus Kaistella, for which the name Kaistella soli sp. nov. is proposed. The type strain is DKR-2^T (=KACC 22070^T=NBRC 114725^T).

Keywords: Kaistella soli sp. nov; Oil-contaminated soil; Phylogeny; Weeksellaceae; Whole genome sequence.