High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems

Conary Meyer; Chuqing Zhou; Zecong Fang; Marjorie L Longo; Tingrui Pan; Cheemeng Tan

doi:10.1007/978-1-0716-1998-8_7

High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems

Methods Mol Biol. 2022:2433:121-134. doi: 10.1007/978-1-0716-1998-8_7.

Authors

Conary Meyer¹, Chuqing Zhou¹, Zecong Fang^{1

2

3}, Marjorie L Longo⁴, Tingrui Pan^{1

2

3

5

6}, Cheemeng Tan⁷

Affiliations

¹ Department of Biomedical Engineering, University of California, Davis, CA, USA.
² Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
³ Shenzhen Engineering Laboratory of Single-Molecule Detection and Instrument Development, Shenzhen, China.
⁴ Department of Chemical Engineering, University of California, Davis, CA, USA.
⁵ Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou, China.
⁶ Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, China.
⁷ Department of Biomedical Engineering, University of California, Davis, CA, USA. cmtan@ucdavis.edu.

Abstract

Cell-free protein synthesis can enable the combinatorial screening of many different components and concentrations. However, manual pipetting methods are unfit to handle many cell-free reactions. Here, we describe a microfluidic method that can generate hundreds of unique submicroliter scale reactions. The method is coupled with a high yield cell-free system that can be applied for broad protein screening assays.

Keywords: Automation; Cell-free protein synthesis; Droplet printing; High throughput; Low-volume liquid handling; Microfluidic adaptive printing; Protein screening.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biological Assay
Cell-Free System
High-Throughput Screening Assays / methods
Microfluidic Analytical Techniques*
Microfluidics* / methods

Abstract

Publication types

MeSH terms

Grants and funding