Decidua Basalis: An Ex Vivo Model to Study HIV-1 Infection During Pregnancy and Beyond

Nabila Jabrane-Ferrat; Hicham El Costa

doi:10.1007/978-1-0716-1871-4_15

Decidua Basalis: An Ex Vivo Model to Study HIV-1 Infection During Pregnancy and Beyond

Methods Mol Biol. 2022:2407:205-213. doi: 10.1007/978-1-0716-1871-4_15.

Authors

Nabila Jabrane-Ferrat¹, Hicham El Costa²

Affiliations

¹ Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), INSERM-CNRS-University Toulouse III, Toulouse, France. nabila.jabrane-ferrat@inserm.fr.
² Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), INSERM-CNRS-University Toulouse III, Toulouse, France. hicham.el-costa@inserm.fr.

PMID: 34985667
DOI: 10.1007/978-1-0716-1871-4_15

Abstract

The human decidua basalis, main uterine mucosa during pregnancy, provides an ex vivo model for studying natural protection of macrophages against HIV-1 infection at the mucosal level. Beyond pregnancy, the decidua constitutes also a valuable tool to assess tissue-resident macrophage infection. Here, we provide a detailed protocol for decidual macrophage purification and tissue infection.

Keywords: Decidua basalis; Decidual Macrophages (dM); Human Immunodeficiency Virus-1 (HIV-1); Infection; Pregnancy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Decidua
Female
HIV Infections*
HIV-1*
Humans
Macrophages
Pregnancy