Hematopoietic Stem and Progenitor Cells (HSPCs)

Valeri H Terry; Gretchen E Zimmerman; Maria C Virgilio; Mark M Painter; Dale Bixby; Kathleen L Collins

doi:10.1007/978-1-0716-1871-4_11

Hematopoietic Stem and Progenitor Cells (HSPCs)

Methods Mol Biol. 2022:2407:115-154. doi: 10.1007/978-1-0716-1871-4_11.

Authors

Valeri H Terry¹, Gretchen E Zimmerman¹, Maria C Virgilio^{2

3}, Mark M Painter⁴, Dale Bixby¹, Kathleen L Collins^{5

6}

Affiliations

¹ Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
² Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA.
³ Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
⁴ Graduate Program in Immunology, University of Michigan, Ann Arbor, MI, USA.
⁵ Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA. klcollin@med.umich.edu.
⁶ Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA. klcollin@med.umich.edu.

PMID: 34985663
DOI: 10.1007/978-1-0716-1871-4_11

Abstract

Cord blood is a readily available source of hematopoietic stem and progenitor cells (HSPCs) which can be infected with HIV-1 in vitro to produce inducible latently infected cells for reactivation studies. Infected HSPCs can also be found in the setting of clinically undetectable viremia in vivo. Here we describe an in vitro infection model utilizing cord blood derived HSPCs, as well as methods for isolating and characterizing provirus from bone marrow HSPCs from suppressed patients.

Keywords: HIV-1; HSPC; Latency; Reactivation; Reservoir.

MeSH terms

Fetal Blood
HIV-1*
Hematopoietic Stem Cells*
Humans
Proviruses
Viremia