Abstract
Maintenance of the energy balance is indispensable for cell survival and function. Adenylate kinase (Ak) is a ubiquitous enzyme highly conserved among many organisms. Ak plays an essential role in energy regulation by maintaining adenine nucleotide homeostasis in cells. However, its role at the whole organism level, especially in animal behavior, remains unclear. Here, we established a model using medaka fish (Oryzias latipes) to examine the function of Ak in environmental adaptation. Medaka overexpressing the major Ak isoform Ak1 exhibited increased locomotor activity compared to that of the wild type. Interestingly, this increase was temperature dependent. Our findings suggest that cellular energy balance can modulate locomotor activity.
MeSH terms
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Adenylate Kinase / classification
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Adenylate Kinase / genetics
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Adenylate Kinase / metabolism*
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Animals
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Fish Proteins / classification
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Fish Proteins / genetics
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Fish Proteins / metabolism*
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Larva / physiology
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Locomotion / physiology*
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Oryzias / growth & development
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Oryzias / metabolism*
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Phylogeny
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Protein Isoforms / classification
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Temperature
Substances
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Fish Proteins
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Protein Isoforms
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Adenylate Kinase
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adenylate kinase 1
Grants and funding
This work was supported by a JSPS KAKENHI “Grant-in-Aid for Scientific Research (S)” (19H05643) for T.Y. and the Sasakawa Scientific Research Grant from The Japan Science Society for M.M. There was no additional external funding received for this study. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.