Isolation and Molecular Characterization of Fowl Adenovirus and Avian Reovirus from Breeder Chickens in Japan in 2019-2021

Makiko Yamaguchi; Yu Miyaoka; Md Amirul Hasan; Md Humayun Kabir; Dany Shoham; Harumi Murakami; Kazuaki Takehara

doi:10.1292/jvms.21-0616

Isolation and Molecular Characterization of Fowl Adenovirus and Avian Reovirus from Breeder Chickens in Japan in 2019-2021

J Vet Med Sci. 2022 Feb 23;84(2):238-243. doi: 10.1292/jvms.21-0616. Epub 2021 Dec 30.

Authors

Makiko Yamaguchi¹, Yu Miyaoka², Md Amirul Hasan², Md Humayun Kabir², Dany Shoham³, Harumi Murakami^{1

2}, Kazuaki Takehara^{1

2}

Affiliations

¹ Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
² Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
³ Bar-Ilan University, Begin-Sadat Center for Strategic Studies, Ramat Gan, 5290002, Israel.

Abstract

Fowl adenoviruses (FAdVs) and avian reoviruses (ARVs) are ubiquitous in poultry farms and most of them are not pathogenic, yet often cause damage to chicks. A total of 104 chicken fecal samples were collected from 7 farms of breeder chickens (layers and broilers) in Japan from 2019 to 2021, and yielded 26 FAdV plus 14 ARV isolates. By sequencing, FAdV isolates were classified as FAdV-1, 5 and 8b. ARV isolates were classified as genotype II, IV and V. These results suggest that FAdVs and ARVs are resident in the breeder chicken farms in Japan.

Keywords: avian reovirus; fowl adenovirus; phylogenetic analysis; virus isolation.

MeSH terms

Adenoviridae Infections* / epidemiology
Adenoviridae Infections* / pathology
Adenoviridae Infections* / veterinary
Animals
Aviadenovirus* / genetics
Chickens
Japan / epidemiology
Orthoreovirus, Avian* / genetics
Phylogeny
Poultry Diseases*