Microchemistry, i.e., the chemistry performed at the scale of a microgram or less, has its roots in the late eighteenth and early nineteenth centuries. In the first half of the twentieth century a wide range of spot tests have been developed. For didactic reasons, they are still part of the curriculum of chemistry students. However, they are even highly important for applied analyses in conservation of cultural heritage, food science, forensic science, clinical and pharmacological sciences, geochemistry, and environmental sciences. Modern pregnancy tests, virus tests, etc. are the most recent examples of sophisticated spot tests. The present ChemTexts contribution aims to provide an overview of the past and present of this analytical methodology.
Keywords: Chemical microscopy; Dipstick immunoassay; ELISA; Lab-on-a-chip; Microchemistry; Microfluidics; Multiplexed tests; Smartphone spectroscopy; Spot tests; µPAD.
© The Author(s), under exclusive licence to Springer Nature Switzerland AG 2021.