Chondroitin sulfate zinc with antibacterial properties and anti-inflammatory effects for skin wound healing

Guofeng Wu; Fenbo Ma; Yizhebang Xue; Ying Peng; Liqiu Hu; Xiaowo Kang; Qili Sun; Dong Fang Ouyang; Bin Tang; Lijun Lin

doi:10.1016/j.carbpol.2021.118996

Chondroitin sulfate zinc with antibacterial properties and anti-inflammatory effects for skin wound healing

Carbohydr Polym. 2022 Feb 15:278:118996. doi: 10.1016/j.carbpol.2021.118996. Epub 2021 Dec 11.

Authors

Guofeng Wu¹, Fenbo Ma², Yizhebang Xue², Ying Peng², Liqiu Hu², Xiaowo Kang², Qili Sun², Dong Fang Ouyang³, Bin Tang⁴, Lijun Lin⁵

Affiliations

¹ Department of Joint and Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China.
² Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China.
³ Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China; Department of Mechanical Engineering, University of Toronto, Canada.
⁴ Department of Joint and Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China; Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, PR China. Electronic address: tangb@sustech.edu.cn.
⁵ Department of Joint and Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China. Electronic address: gost1@smu.edu.cn.

PMID: 34973799
DOI: 10.1016/j.carbpol.2021.118996

Abstract

A chondroitin sulfate zinc (CSZn) complex was prepared by an ion-exchange method. The purified product was characterized by energy-dispersive X-ray spectroscopy, high-performance chromatography, elemental analysis, Fourier transform infrared spectroscopy, inductively coupled mass spectrometry, and nuclear magnetic resonance spectroscopy. The CSZn demonstrated antibacterial activity against Escherichia coli and Staphylococcus aureus and satisfied MTT cell viability (NIH3T3 fibroblasts) at ≤50 μg/mL. RT-PCR demonstrated significant promotion by CSZn of fibroblast growth factor beta (β-FGF), collagen III (COLIIIα1), vascular endothelial growth factor (VEGF) and reduction of cytokines IL-6, IL-1β & TNF-alpha. An in vivo rat full-thickness wound healing model demonstrated significant wound healing of CSZn relative to controls of saline treatment, zinc chloride treatment and chondroitin treatment. CSZn has demonstrated promising antibacterial and wound healing properties making it deserving of consideration for more advanced wound healing applications.

Keywords: Anti-inflammatory; Chondroitin sulfate zinc; Wound healing.

MeSH terms

Animals
Anti-Bacterial Agents / chemical synthesis
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Anti-Inflammatory Agents, Non-Steroidal / chemical synthesis
Anti-Inflammatory Agents, Non-Steroidal / chemistry
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Carbohydrate Conformation
Cell Survival / drug effects
Chondroitin Sulfates / chemistry
Chondroitin Sulfates / pharmacology*
Dose-Response Relationship, Drug
Escherichia coli / drug effects
Formaldehyde
Male
Microbial Sensitivity Tests
Particle Size
Rats
Rats, Sprague-Dawley
Skin / drug effects*
Skin / pathology
Staphylococcus aureus / drug effects
Wound Healing / drug effects*
Zinc / chemistry
Zinc / pharmacology*

Substances

Anti-Bacterial Agents
Anti-Inflammatory Agents, Non-Steroidal
Formaldehyde
Chondroitin Sulfates
Zinc