Metabolomic profiling of M. speciosa champ at different growth stages

Maoyuan Wang; Mei Zhang; Qing Yang; Qinglong Wang; Bokai Ma; Zhiying Li; Wen Cheng; Huan Tang; Shixiu Feng; Zhunian Wang

doi:10.1016/j.foodchem.2021.131941

Metabolomic profiling of M. speciosa champ at different growth stages

Food Chem. 2021 Dec 23:376:131941. doi: 10.1016/j.foodchem.2021.131941. Online ahead of print.

Authors

Maoyuan Wang¹, Mei Zhang², Qing Yang¹, Qinglong Wang¹, Bokai Ma³, Zhiying Li¹, Wen Cheng⁴, Huan Tang¹, Shixiu Feng⁵, Zhunian Wang⁶

Affiliations

¹ Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Haikou 571101, China; National Genebank of Tropical Crops, Tropical Wild Plant Gene Resource, Ministry of Agriculture, Danzhou, Haikou 571737, China; Hainan Provincial Engineering Research Center for Tropical Medicinal Plants, Danzhou, Haikou 571737, China.
² Institute of Analysis and Testing, Beijing Academy of Science and Technology (Beijing Center for Physical & Chemical Analysis), Beijing 100089, China. Electronic address: zhangmei@bcpca.ac.cn.
³ Institute of Analysis and Testing, Beijing Academy of Science and Technology (Beijing Center for Physical & Chemical Analysis), Beijing 100089, China.
⁴ Key Laboratory of South Subtropical Plant Diversity, Fairy Lake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen 518004, China. Electronic address: chengwen@szbg.ac.cn.
⁵ Key Laboratory of South Subtropical Plant Diversity, Fairy Lake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen 518004, China. Electronic address: fengshixiu@scbg.ac.cn.
⁶ Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Haikou 571101, China; National Genebank of Tropical Crops, Tropical Wild Plant Gene Resource, Ministry of Agriculture, Danzhou, Haikou 571737, China; Hainan Provincial Engineering Research Center for Tropical Medicinal Plants, Danzhou, Haikou 571737, China. Electronic address: zhnwangCATAS@163.com.

PMID: 34973642
DOI: 10.1016/j.foodchem.2021.131941

Abstract

Millettia speciosa Champ (M. speciosa) is an edible food and folk medicine and extracts from its roots exhibit a hepatoprotective effect. However, its metabolic growth process and the best harvest time have not been reported. This study systematically evaluated the metabolomic profiling of M. speciosa root extracts at different growth stages through the UPLC-Q-TOF-MS, nuclear magnetic resonance (NMR) and An orthogonal partial least squares-discriminant analysis (OPLS-DA). The results revealed there were significant differences among extracts from six ages of M. speciosa, and 110 compounds were identified. Pharmacological studies showed that 5-year and 20-year old M. speciosa roots may exhibit higher fat-lowering effects, while 5-year-old (M.s-5Y) showed better hepatoprotective activity in high-fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD) mice. Hence, our study suggested that M.s-5Y may have potent efficacy in ameliorating NAFLD, which might be useful in determining the optimum time to harvest M. speciosa.

Keywords: Growth stages; Millettia speciosa Champ; NMR; Nonalcoholic fatty liver disease; UPLC-Q-TOF-MS metabolomics.