Recapitulating human myogenesis ex vivo using human pluripotent stem cells

Exp Cell Res. 2022 Feb 15;411(2):112990. doi: 10.1016/j.yexcr.2021.112990. Epub 2021 Dec 30.

Abstract

Human pluripotent stem cells (hPSCs) provide a human model for developmental myogenesis, disease modeling and development of therapeutics. Differentiation of hPSCs into muscle stem cells has the potential to provide a cell-based therapy for many skeletal muscle wasting diseases. This review describes the current state of hPSCs towards recapitulating human myogenesis ex vivo, considerations of stem cell and progenitor cell state as well as function for future use of hPSC-derived muscle cells in regenerative medicine.

Keywords: cell differentiation; development; human myogenesis; human pluripotent stem cells; muscle stem and progenitor cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Cell Differentiation / physiology
  • Humans
  • Models, Biological
  • Muscle Development / genetics
  • Muscle Development / physiology*
  • Muscle, Skeletal / cytology
  • Muscle, Skeletal / physiology
  • Myoblasts, Skeletal / cytology
  • Myoblasts, Skeletal / physiology
  • PAX7 Transcription Factor / genetics
  • PAX7 Transcription Factor / metabolism
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / physiology*
  • Satellite Cells, Skeletal Muscle / cytology
  • Satellite Cells, Skeletal Muscle / physiology

Substances

  • PAX7 Transcription Factor
  • PAX7 protein, human