Extraction of Metabolites from Cancer Cells

Elena Kochetkova; Tina Becirovic; Erik Norberg

doi:10.1007/978-1-0716-2071-7_20

Extraction of Metabolites from Cancer Cells

Methods Mol Biol. 2022:2445:329-335. doi: 10.1007/978-1-0716-2071-7_20.

Authors

Elena Kochetkova^{1

2}, Tina Becirovic¹, Erik Norberg³

Affiliations

¹ Department of Physiology and Pharmacology, Biomedicum, Karolinska Institutet, Stockholm, Sweden.
² Institute of Cytology, Russian Academy of Sciences, Saint-Petersburg, Russia.
³ Department of Physiology and Pharmacology, Biomedicum, Karolinska Institutet, Stockholm, Sweden. Erik.Norberg@ki.se.

PMID: 34973001
DOI: 10.1007/978-1-0716-2071-7_20

Abstract

Cancer cells possess an elevated demand for nutrients and metabolites due to their uncontrolled proliferation and need to survive in unfavorable conditions. Autophagy is a conservative degradation pathway that counters lack of nutrients and provides organelle and protein quality control, beyond maintenance of cellular metabolism.Mass spectrometry-based metabolomics is a powerful tool to study the metabolome of a cell. Such analysis requires proper sample preparation including the extraction of metabolites. Here, we provide a protocol for the extraction of metabolites from adherent cancer cells suitable for global metabolome profiling by mass spectrometry.

Keywords: Autophagy; CE-MS; Cancer metabolism; Chaperone-mediated autophagy; GC-MS; LC-MS; Mass spectrometry; Metabolism; Methanol extraction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Mass Spectrometry
Metabolome*
Metabolomics / methods
Neoplasms*